##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2079228_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3435180 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.227439610151432 31.0 30.0 34.0 26.0 34.0 2 30.40188781956113 31.0 31.0 34.0 26.0 34.0 3 30.55625585849941 31.0 31.0 34.0 26.0 34.0 4 34.07779039235208 37.0 35.0 37.0 30.0 37.0 5 34.10499508031602 37.0 35.0 37.0 30.0 37.0 6 33.7314033034659 37.0 35.0 37.0 28.0 37.0 7 33.42682974400177 37.0 35.0 37.0 27.0 37.0 8 33.38278372603474 37.0 35.0 37.0 27.0 37.0 9 34.72953557018846 39.0 35.0 39.0 27.0 39.0 10 34.5890043607613 39.0 35.0 39.0 27.0 39.0 11 34.51013542230684 39.0 35.0 39.0 26.0 39.0 12 34.32935071815742 39.0 35.0 39.0 25.0 39.0 13 34.15538254181731 38.0 34.0 39.0 25.0 39.0 14 35.25174401341415 39.0 35.0 41.0 25.0 41.0 15 35.168846465105176 39.0 34.0 41.0 25.0 41.0 16 35.18412106498058 39.0 35.0 41.0 25.0 41.0 17 35.141936375968655 39.0 34.0 41.0 24.0 41.0 18 35.17082190744008 39.0 35.0 41.0 25.0 41.0 19 35.21085823741405 39.0 35.0 41.0 24.0 41.0 20 35.23964304636147 39.0 35.0 41.0 25.0 41.0 21 35.1429893047817 39.0 34.0 41.0 24.0 41.0 22 35.13735612107663 39.0 35.0 41.0 24.0 41.0 23 35.12449944398838 39.0 35.0 41.0 24.0 41.0 24 35.112073603129964 39.0 35.0 41.0 24.0 41.0 25 35.05773991464785 39.0 34.0 41.0 24.0 41.0 26 34.98806991191146 39.0 34.0 41.0 23.0 41.0 27 34.91083174680803 39.0 34.0 41.0 23.0 41.0 28 34.879460173848244 39.0 34.0 41.0 23.0 41.0 29 34.82371840776903 39.0 34.0 41.0 23.0 41.0 30 34.73997403338399 39.0 34.0 41.0 22.0 41.0 31 34.622972013111394 39.0 34.0 41.0 21.0 41.0 32 34.56406098079285 39.0 34.0 41.0 21.0 41.0 33 34.438034688138615 39.0 34.0 41.0 19.0 41.0 34 34.44155240773409 39.0 34.0 41.0 19.0 41.0 35 34.29356831374193 39.0 34.0 41.0 18.0 41.0 36 34.23815957242415 39.0 34.0 41.0 18.0 41.0 37 34.145491357075905 39.0 34.0 41.0 16.0 41.0 38 34.095116122008164 39.0 33.0 41.0 16.0 41.0 39 34.08534399944108 39.0 33.0 41.0 16.0 41.0 40 34.0452232488545 39.0 33.0 41.0 16.0 41.0 41 33.93761462281452 39.0 33.0 41.0 15.0 41.0 42 33.849767697762566 39.0 33.0 41.0 15.0 41.0 43 33.753155875383534 38.0 33.0 41.0 15.0 41.0 44 33.62015702233944 38.0 33.0 41.0 9.0 41.0 45 33.52616718774562 38.0 33.0 41.0 9.0 41.0 46 33.437802094795614 38.0 33.0 40.0 9.0 41.0 47 33.248462962639515 38.0 33.0 40.0 8.0 41.0 48 33.140640665117985 38.0 33.0 40.0 7.0 41.0 49 33.01808842622512 38.0 33.0 40.0 2.0 41.0 50 32.94370949993887 38.0 32.0 40.0 2.0 41.0 51 32.841007458124466 38.0 32.0 40.0 2.0 41.0 52 32.78070086574794 38.0 32.0 40.0 2.0 41.0 53 32.6737748822478 38.0 32.0 40.0 2.0 41.0 54 32.59781699940032 38.0 32.0 40.0 2.0 41.0 55 32.493748508084 38.0 32.0 40.0 2.0 41.0 56 32.44756082650691 38.0 32.0 40.0 2.0 41.0 57 32.289634313194654 38.0 32.0 40.0 2.0 41.0 58 32.18885036591969 37.0 31.0 40.0 2.0 41.0 59 32.06939345245373 37.0 31.0 40.0 2.0 41.0 60 31.95151287559895 37.0 31.0 40.0 2.0 41.0 61 31.81113653433008 37.0 31.0 40.0 2.0 41.0 62 31.6561114701413 37.0 31.0 40.0 2.0 41.0 63 31.51166285318382 36.0 31.0 40.0 2.0 41.0 64 31.336967495153093 36.0 31.0 40.0 2.0 41.0 65 31.177693454200362 36.0 31.0 39.0 2.0 41.0 66 30.994957178372022 36.0 30.0 39.0 2.0 41.0 67 30.816174989374648 36.0 30.0 39.0 2.0 41.0 68 30.605236697931403 35.0 30.0 39.0 2.0 41.0 69 30.453541590251458 35.0 30.0 39.0 2.0 41.0 70 30.268037773857557 35.0 30.0 39.0 2.0 40.0 71 30.105701011300717 35.0 30.0 39.0 2.0 40.0 72 29.890579532950238 35.0 30.0 38.0 2.0 40.0 73 29.660729277650663 35.0 30.0 37.0 2.0 40.0 74 29.409410860566258 35.0 29.0 37.0 2.0 40.0 75 29.20238823001997 35.0 29.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1395959370695863 1101 2 1.2216930593474054 1101 3 1.2164808116697117 1101 4 1.1897601722170066 1101 5 1.1729215851093358 1101 6 1.3440345190944925 1101 7 1.4427330884844949 1101 8 1.5279513499361386 1101 9 1.849108392877028 1101 10 1.8129705433896817 1101 11 1.8961604372474739 1101 12 2.0083003037524207 1101 13 2.1407361060434766 1101 14 2.219360755104944 1101 15 2.4158510169414456 1101 16 2.3237029586615634 1101 17 2.300707220987654 1101 18 2.3644733436909675 1101 19 2.362204527019074 1101 20 2.241272000348829 1101 21 2.3399100913344384 1101 22 2.2874312766557736 1101 23 2.2457220750896596 1101 24 2.3001961280689684 1101 25 2.3157946375200424 1101 26 2.339899839732894 1101 27 2.3115029496268136 1101 28 2.271742886705205 1101 29 2.355514615018123 1101 30 2.3331279235149864 1101 31 2.340405224349425 1101 32 2.293961441542109 1101 33 2.4003785624324365 1101 34 2.2889090797826483 1101 35 2.37990763070691 1101 36 2.469404567011118 1101 37 2.406261373398806 1101 38 2.3876466924486834 1101 39 2.3199974110351036 1101 40 2.2531771214997036 1101 41 2.322269716410645 1101 42 2.4249520795346484 1101 43 2.360451209461324 1101 44 2.4450005112797655 1101 45 2.3323846018905385 1101 46 2.355859890546242 1101 47 2.4068351413502995 1101 48 2.439496955972885 1101 49 2.508499007718889 1101 50 2.529479585843852 1101 51 2.5275942368923054 1101 52 2.4115924418831725 1101 53 2.490960542437172 1101 54 2.557255393017087 1101 55 2.556763019728585 1101 56 2.4590879226815545 1101 57 2.5941254324242067 1101 58 2.615899707813732 1101 59 2.4664321553741004 1101 60 2.570562598340846 1101 61 2.593948019979667 1101 62 2.544487061584192 1101 63 2.6296218320409714 1101 64 2.6630196623503437 1101 65 2.5992946310113787 1101 66 2.6700699098347265 1101 67 2.6445027015960925 1101 68 2.6748453614494814 1101 69 2.6767773831755655 1101 70 2.7515687906734527 1101 71 2.659237737589134 1101 72 2.590840312533807 1101 73 2.6146427893731357 1101 74 2.573136129486951 1101 75 2.608202330647483 1102 1 0.8577707155769083 1102 2 0.8770588337155552 1102 3 0.8098694104027295 1102 4 0.7797489245573033 1102 5 0.7576653449124748 1102 6 0.9280471019194252 1102 7 0.9698093771997378 1102 8 1.0256467661588715 1102 9 1.291533861311997 1102 10 1.357966995868324 1102 11 1.490578133565407 1102 12 1.371824507181607 1102 13 1.5109977921008877 1102 14 1.5594050734152987 1102 15 1.6755449651152077 1102 16 1.6458423790195127 1102 17 1.539473403340537 1102 18 1.663339283768309 1102 19 1.6450232522368538 1102 20 1.4479539961044594 1102 21 1.6228840848784998 1102 22 1.5738474732857455 1102 23 1.5969878322936566 1102 24 1.5750964059383676 1102 25 1.7092488476013301 1102 26 1.64297851086382 1102 27 1.5502423970360937 1102 28 1.4603065795444294 1102 29 1.6390864272122627 1102 30 1.5902342376284295 1102 31 1.681308699338416 1102 32 1.682314260536259 1102 33 1.7793333764228834 1102 34 1.6620521283413794 1102 35 1.7021286796500448 1102 36 1.6329044479694304 1102 37 1.7842555486613918 1102 38 1.8638003487686134 1102 39 1.79667805422406 1102 40 1.6682562624695763 1102 41 1.7816134210037546 1102 42 1.8548090001279078 1102 43 1.7110778195891214 1102 44 1.8458383116425097 1102 45 1.8548264514107728 1102 46 1.7516072152721947 1102 47 1.816159704356572 1102 48 1.783171017461676 1102 49 1.8285874307764374 1102 50 1.8201509888668 1102 51 1.8513042956379948 1102 52 1.8312029580495093 1102 53 1.869747748896586 1102 54 1.938914153892 1102 55 1.8936787564067927 1102 56 1.898403634681351 1102 57 1.8990305826975273 1102 58 1.8867274153397773 1102 59 1.8723620924204312 1102 60 1.8742293195847672 1102 61 1.8934283496262658 1102 62 1.8667912419643784 1102 63 1.900215964749119 1102 64 1.918482093822277 1102 65 1.8765233800928591 1102 66 1.9599447222748587 1102 67 1.8908045786580665 1102 68 1.7861132701045612 1102 69 1.869770138638632 1102 70 1.884750810553811 1102 71 1.9156696667051456 1102 72 1.9486417351808392 1102 73 2.0087745483505017 1102 74 1.9994787101050662 1102 75 1.9755306476195038 1103 1 0.716740516427329 1103 2 0.7846184663030975 1103 3 0.7543465568738412 1103 4 0.6674446698199645 1103 5 0.7102942881197762 1103 6 0.8441151024598739 1103 7 0.8673284116237951 1103 8 0.9242890056797393 1103 9 1.1827411355953856 1103 10 1.1961930673227457 1103 11 1.2794024330695493 1103 12 1.3581210130373123 1103 13 1.3855927442606273 1103 14 1.4945230612515275 1103 15 1.5569976590781849 1103 16 1.6459393322666074 1103 17 1.6655941298466033 1103 18 1.5853894495226228 1103 19 1.5743212784485792 1103 20 1.5156165259455392 1103 21 1.5801293168768993 1103 22 1.592983577199334 1103 23 1.5938173112454663 1103 24 1.5274035934826102 1103 25 1.6082339396273753 1103 26 1.4949922332644263 1103 27 1.5784963716580904 1103 28 1.6385052643004272 1103 29 1.4888714930459415 1103 30 1.6018861503396096 1103 31 1.5240534146371019 1103 32 1.5792682915548468 1103 33 1.5540072284664603 1103 34 1.621029376492345 1103 35 1.66689453237057 1103 36 1.762064964272561 1103 37 1.7532193271127312 1103 38 1.7419845308866968 1103 39 1.6829503967021253 1103 40 1.603487343213537 1103 41 1.6764717170890577 1103 42 1.761292578484344 1103 43 1.6639840191094493 1103 44 1.6115277910277825 1103 45 1.570165031517213 1103 46 1.531541789699311 1103 47 1.67754284560489 1103 48 1.6924130943869855 1103 49 1.755171457769329 1103 50 1.7203200402836103 1103 51 1.6967411063303786 1103 52 1.7227254168484123 1103 53 1.758483118870636 1103 54 1.80295215403126 1103 55 1.9077601491764966 1103 56 1.8166378082454173 1103 57 1.828781787523198 1103 58 1.7752174624721988 1103 59 1.7461038329491032 1103 60 1.7889136900757485 1103 61 1.8024317901407763 1103 62 1.8816658990542336 1103 63 1.7607389773689484 1103 64 1.6928673917903865 1103 65 1.719600572400605 1103 66 1.8546915394185 1103 67 1.9449578666989034 1103 68 1.9551805960071924 1103 69 1.9210716639287817 1103 70 1.9881020496877824 1103 71 1.8704016076930152 1103 72 1.822922770941254 1103 73 1.889753312707306 1103 74 1.879046061139455 1103 75 1.9256471067993068 1104 1 0.6236278360726963 1104 2 0.5464740365276164 1104 3 0.5230264733017478 1104 4 0.4859244685732378 1104 5 0.5189571234673309 1104 6 0.5218532454736788 1104 7 0.6668431434966635 1104 8 0.5392766848699182 1104 9 0.7550232074290761 1104 10 0.8794834930120388 1104 11 0.9641991164185839 1104 12 0.9991831253454109 1104 13 0.9638431333731532 1104 14 0.9922251268176865 1104 15 1.1400582875978529 1104 16 1.2565771861615147 1104 17 1.1297821767420686 1104 18 1.0169481241887794 1104 19 1.125683274758856 1104 20 1.0836384630607796 1104 21 1.1151943080773847 1104 22 1.1196802177338867 1104 23 1.119511817017596 1104 24 1.0547525121838959 1104 25 1.0358860624099364 1104 26 1.0842844378602905 1104 27 1.1105183296402714 1104 28 1.0450996642846277 1104 29 1.1074114705618143 1104 30 1.1065412664100975 1104 31 1.0653167811227888 1104 32 0.851802257156443 1104 33 1.0424104269211156 1104 34 1.0687977132221462 1104 35 1.1428979992634964 1104 36 1.0699938050283748 1104 37 1.1976872220223527 1104 38 1.1419624342884234 1104 39 0.9796824476875088 1104 40 0.985393657363069 1104 41 1.0719691532502011 1104 42 1.0325749748734694 1104 43 1.1396113966829304 1104 44 1.2300402316361883 1104 45 1.083404023927237 1104 46 1.0822766599572589 1104 47 1.1656421692552144 1104 48 1.1268146287032295 1104 49 1.1006939528888822 1104 50 1.1067807875212523 1104 51 1.2447076417216394 1104 52 1.1275593923990712 1104 53 1.1046852054900498 1104 54 1.1879113014081213 1104 55 1.1833165046313994 1104 56 1.163366561509136 1104 57 1.285386378771527 1104 58 1.2293779761864343 1104 59 1.2245707538198047 1104 60 1.074113366191039 1104 61 1.2576748881211195 1104 62 1.2996900160193903 1104 63 1.240111150783644 1104 64 1.2985741270794762 1104 65 1.2804263591680574 1104 66 1.3213899909052849 1104 67 1.253029683251679 1104 68 1.3886352184255166 1104 69 1.334709662029148 1104 70 1.3099029708886292 1104 71 1.2966914546973634 1104 72 1.264036789760997 1104 73 1.2583986496357156 1104 74 1.2660056400148108 1104 75 1.2742547043774017 1105 1 0.5654101228837725 1105 2 0.5859501356044845 1105 3 0.5542510414399437 1105 4 0.4736616041184334 1105 5 0.5207097968975418 1105 6 0.6532533003132741 1105 7 0.8037600615114684 1105 8 0.8018014997862934 1105 9 0.9150038307724628 1105 10 0.8919488983680353 1105 11 1.0731434542304896 1105 12 1.1150295744816887 1105 13 1.1955839248912952 1105 14 1.2418023135465077 1105 15 1.2878736609640882 1105 16 1.3519373908959977 1105 17 1.454386874667307 1105 18 1.255141342358968 1105 19 1.367428636243183 1105 20 1.2016795927564203 1105 21 1.2867965382208837 1105 22 1.2894932147177087 1105 23 1.2138139831815664 1105 24 1.190652338525183 1105 25 1.3298858796112896 1105 26 1.296777628610684 1105 27 1.3411133392372037 1105 28 1.3301737891361043 1105 29 1.3012741887776968 1105 30 1.3650986194856856 1105 31 1.2329193768635776 1105 32 1.294775294355965 1105 33 1.417940999994876 1105 34 1.3266966255702002 1105 35 1.3956558824551593 1105 36 1.44159237919893 1105 37 1.464686947824383 1105 38 1.52933433813633 1105 39 1.3914166584543537 1105 40 1.3693516136741977 1105 41 1.4497715479124764 1105 42 1.4596840142665783 1105 43 1.4504758514859617 1105 44 1.2976731719524253 1105 45 1.321572747078683 1105 46 1.265299783986137 1105 47 1.3155823940975537 1105 48 1.4060304225063547 1105 49 1.4062386014584831 1105 50 1.372444620808885 1105 51 1.4373404906385545 1105 52 1.4215771238678556 1105 53 1.41960523108186 1105 54 1.4304035781652686 1105 55 1.5089295198950836 1105 56 1.3629081025017342 1105 57 1.5518490421478148 1105 58 1.6252463611603858 1105 59 1.6141311230730722 1105 60 1.5031966309748803 1105 61 1.507057577089224 1105 62 1.508550997648129 1105 63 1.538712741131878 1105 64 1.5727139680446527 1105 65 1.6247864725032208 1105 66 1.6349317146965312 1105 67 1.6210688021621955 1105 68 1.688919287523401 1105 69 1.6298669602651437 1105 70 1.6915554706601306 1105 71 1.7390312773826757 1105 72 1.7032785409720397 1105 73 1.7241732589036083 1105 74 1.727536395887217 1105 75 1.7692639357090876 1106 1 0.4273145271539285 1106 2 0.4617056565886948 1106 3 0.4408060545830139 1106 4 0.5109146842464654 1106 5 0.4051915307101055 1106 6 0.5955606872560324 1106 7 0.596201024979095 1106 8 0.6326751653621727 1106 9 0.6460493040124575 1106 10 0.6575882238691761 1106 11 0.8004134504753253 1106 12 0.9540952898403035 1106 13 0.9588400449134653 1106 14 1.0503949856134582 1106 15 1.0145023468357337 1106 16 1.0509179892186893 1106 17 1.0846463385730374 1106 18 0.9773859286651359 1106 19 1.0334514175675054 1106 20 0.9291841257750306 1106 21 1.0096261432134597 1106 22 1.0899210887587998 1106 23 1.0673433002782886 1106 24 0.9471811370517287 1106 25 0.9305250441768464 1106 26 0.9586758815656395 1106 27 0.9094122502398321 1106 28 0.9978885397753743 1106 29 0.9358117493520552 1106 30 1.0080875864581245 1106 31 0.8763287075504138 1106 32 0.9309356577127232 1106 33 1.006258150362342 1106 34 0.8246953738490816 1106 35 0.8399762515340683 1106 36 0.821005500812575 1106 37 0.9024508016948829 1106 38 0.9572153888667998 1106 39 0.8702294546205067 1106 40 0.8253222876366095 1106 41 0.916963794394249 1106 42 0.905563169533643 1106 43 0.9335864857509364 1106 44 1.005596280512414 1106 45 0.9308728203937804 1106 46 0.9061082527735778 1106 47 0.8786049787228407 1106 48 0.8265443434333406 1106 49 0.9098973465186546 1106 50 0.9442633000642928 1106 51 0.9375982021347227 1106 52 0.8951936322812131 1106 53 0.9682145689213897 1106 54 0.9747015593179285 1106 55 0.9965268233301643 1106 56 0.9273958445756563 1106 57 0.9639620546829093 1106 58 0.8811985096502752 1106 59 0.9100301270606934 1106 60 0.9288247267494292 1106 61 0.9366180799234733 1106 62 1.0040475911785194 1106 63 0.860653624131718 1106 64 0.9294939691175514 1106 65 0.924170768767727 1106 66 0.9700142388815678 1106 67 0.9751279906892769 1106 68 0.9001682548534831 1106 69 0.9520340829159295 1106 70 0.9199967202354387 1106 71 0.9206914172178031 1106 72 0.9491016906600009 1106 73 0.928060389422356 1106 74 0.9109184748171728 1106 75 0.9185122235334191 1107 1 0.2669884193340515 1107 2 0.31794504828681625 1107 3 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2215 4 -1.121415427211275 2215 5 -1.2604060403498067 2215 6 -1.4592630330024718 2215 7 -1.5184633803588135 2215 8 -1.6539996506701975 2215 9 -2.1515927530402266 2215 10 -1.6873134725574275 2215 11 -1.7247582820088923 2215 12 -4.858465807860387 2215 13 -2.967305796641945 2215 14 -2.9295648298383696 2215 15 -2.3468355761108626 2215 16 -4.447805990460978 2215 17 -2.9336107329461143 2215 18 -2.3532359510777994 2215 19 -2.648692038550557 2215 20 -2.807135225967528 2215 21 -2.488693813072942 2215 22 -3.172591116909288 2215 23 -2.865427137721909 2215 24 -2.6211400163924026 2215 25 -2.6731746864696007 2215 26 -2.7877685391730154 2215 27 -3.7247086038732533 2215 28 -3.314050743381003 2215 29 -4.761968972690806 2215 30 -3.779352134292001 2215 31 -3.021151726272908 2215 32 -4.190790452297332 2215 33 -2.9977020418543 2215 34 -2.951918323327721 2215 35 -2.90878424631153 2215 36 -2.600997731097131 2215 37 -3.529424403698126 2215 38 -4.787556825865288 2215 39 -3.559699987982576 2215 40 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-1.0987347463315196 2216 2 -1.0202255853881397 2216 3 -1.016123204032894 2216 4 -0.805568442829923 2216 5 -0.9893344686960148 2216 6 -1.210964003509666 2216 7 -1.3325296769191155 2216 8 -1.3904077557469066 2216 9 -1.710849958630618 2216 10 -1.5130034825843452 2216 11 -1.574180489431484 2216 12 -1.832036823908652 2216 13 -2.6467876378182957 2216 14 -2.4600246482241204 2216 15 -4.090763495306867 2216 16 -3.57015248167275 2216 17 -3.2543321058826606 2216 18 -2.7872946066685316 2216 19 -3.410730988169963 2216 20 -2.96913730865203 2216 21 -2.8054234483653033 2216 22 -2.3362774090247456 2216 23 -2.281196077180603 2216 24 -3.1021578559751504 2216 25 -3.1832274671358647 2216 26 -2.824475221825267 2216 27 -2.6664922945656144 2216 28 -2.6375633000657714 2216 29 -2.752281813905064 2216 30 -2.8845164039884956 2216 31 -2.8796493689446017 2216 32 -2.6593950611101675 2216 33 -2.912159603626854 2216 34 -2.8434388539737334 2216 35 -2.954460817746046 2216 36 -2.869111846153185 2216 37 -5.864584681954291 2216 38 -3.7504349442245015 2216 39 -3.124669189969527 2216 40 -2.923796608995154 2216 41 -2.625772382335679 2216 42 -2.730188554479227 2216 43 -2.6970546649459592 2216 44 -2.4811142190603164 2216 45 -2.4593152475557893 2216 46 -2.6085137639682543 2216 47 -5.678646982159993 2216 48 -3.897993722362859 2216 49 -3.09843208935305 2216 50 -3.162718357058317 2216 51 -3.1085991471884036 2216 52 -2.8075630505117566 2216 53 -4.343600670997283 2216 54 -3.502331498964171 2216 55 -2.9341502923114895 2216 56 -2.851232742568687 2216 57 -3.1135218498709634 2216 58 -3.143832109664565 2216 59 -2.893568407443361 2216 60 -2.6556900222779696 2216 61 -2.425535313588174 2216 62 -2.2904751592935213 2216 63 -2.3989130133952834 2216 64 -2.8445657100237582 2216 65 -2.4918761700185748 2216 66 -2.929226568334993 2216 67 -2.670960708233512 2216 68 -2.664271859357882 2216 69 -2.5092450451003856 2216 70 -2.542421725852275 2216 71 -2.741277240390634 2216 72 -2.9309562008119983 2216 73 -3.001138612323757 2216 74 -3.215929734226801 2216 75 -3.6685603432982887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80213.0 3 33768.0 4 22415.0 5 16257.0 6 12455.0 7 11417.0 8 11611.0 9 12063.0 10 13051.0 11 13920.0 12 14264.0 13 14696.0 14 14963.0 15 15539.0 16 16164.0 17 16877.0 18 18003.0 19 19554.0 20 21592.0 21 24202.0 22 28487.0 23 32566.0 24 33473.0 25 34500.0 26 37190.0 27 42280.0 28 48316.0 29 54954.0 30 63200.0 31 73531.0 32 88651.0 33 110338.0 34 142111.0 35 189667.0 36 260087.0 37 381173.0 38 570630.0 39 725746.0 40 115256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.813738701351596 20.76646576614923 14.203965127972337 27.21583040452684 2 12.55428319400756 22.08800886173051 37.554523369029646 27.803184575232276 3 15.47709740889776 26.812372805027046 28.57458082970349 29.135948956371706 4 10.43284485820248 18.130782084199375 35.805256202003974 35.63111685559417 5 11.670637360385285 40.105316537648235 32.371691864852025 15.852354237114458 6 30.944036241188183 38.17188879178469 17.099126557311358 13.784948409715764 7 27.23163446993686 33.08912444986031 21.264749858415918 18.414491221786918 8 26.160183759203242 35.48552663373053 19.441706235643814 18.912583371422418 9 26.06617810814139 16.29908329046583 19.594218695005505 38.040519906387274 10 14.057088892666918 30.232770044459144 33.09906307422511 22.611077988648827 11 33.7902778812833 24.24896714424116 23.25782734657376 18.70292762790178 12 22.948087943226895 25.614750339429133 30.129863852741185 21.307297864602788 13 26.889081084983502 22.93318281099306 26.35079293503717 23.826943168986265 14 21.5263303143289 21.732145061261182 27.81238428378797 28.92914034062195 15 22.62843039272569 29.7649514860729 24.52904561959806 23.077572501603353 16 22.239028731270615 29.3083587832592 25.683683839289795 22.768928646180385 17 21.110264636641695 28.661289144066814 27.274833828845967 22.953612390445517 18 21.194056788872782 28.071367439260825 28.81892651913437 21.915649252732024 19 22.03196958493686 27.440744773138796 29.252606880651154 21.274678761273194 20 22.358765213674268 26.928065141518694 29.072873638056212 21.640296006750827 21 22.32516264363348 26.756424580029574 29.00744799087105 21.910964785465893 22 22.089510354339716 26.707116778228084 28.88595583693189 22.317417030500312 23 21.959227755168577 26.713651104163393 28.719251975151227 22.6078691655168 24 21.69743652443249 26.808929954179984 29.020313346025535 22.47332017536199 25 21.90117408968168 26.677965470142396 29.011123751590894 22.409736688585024 26 21.633108493036314 26.902499112855544 29.121975885390544 22.342416508717594 27 21.775045266914688 27.034041884268074 28.871179967279737 22.3197328815375 28 21.747787668583015 26.909705089432073 28.899820882592074 22.442686359392834 29 21.829423785653155 27.020301701803106 28.914816690828427 22.23545782171531 30 22.07537887388725 26.96082883575242 28.707374868274734 22.256417422085597 31 21.946855768838898 26.65478955979017 28.9958313683708 22.402523303000134 32 21.893845446235712 26.97384125431564 28.757881683055906 22.374431616392737 33 21.842057766987466 26.742063006887555 28.815578805186338 22.60030042093864 34 22.04178529218265 26.85748636170448 28.918018851996113 22.182709494116757 35 22.042380336623022 26.962824051621197 28.758888185706827 22.235907426048957 36 22.026911213141325 26.89828793402788 28.847228707467625 22.22757214536317 37 22.05959410474934 26.649349726884125 28.80027550294425 22.490780665422285 38 21.96915092560032 26.891315030146927 28.812584857369757 22.326949186883002 39 22.060212274174862 27.12093689413655 28.598093840788547 22.22075699090004 40 22.20696505189395 26.77254955273073 28.704937937731923 22.3155474576434 41 21.98011570305193 26.922370430834196 28.551109361886578 22.5464045042273 42 22.147013308212806 26.9602511897512 28.349584824282452 22.543150677753545 43 21.967087605307437 26.79082318830454 28.475800394739142 22.76628881164888 44 22.03625428652938 27.094184293108363 28.265622179914878 22.60393924044737 45 22.17470409119755 27.042687719420815 28.135323330946267 22.647284858435366 46 22.265186180982127 26.83362351714423 27.90136991405688 22.999820387816765 47 22.106830842965806 27.113775188353177 27.97742993139493 22.801964037286083 48 22.077591276148556 27.380253727606707 27.796534679405443 22.745620316839293 49 22.23074840546465 27.121889164146168 27.744565771126318 22.90279665926286 50 22.345243969358094 27.317290435830593 27.619051777716646 22.718413817094664 51 22.315690129676412 27.39692928958892 27.406186484146616 22.881194096588054 52 22.30960286589104 27.478209179072195 27.381648958622623 22.830538996414145 53 22.341041808580627 27.29883150227936 27.53977957486944 22.820347114270575 54 22.163800536682523 27.46020730221258 27.640721965499317 22.735270195605583 55 22.11822961242206 27.167979552745418 27.643878923375194 23.069911911457332 56 22.1505487778075 27.53224795563783 27.48214867405745 22.835054592497215 57 22.26847567477561 27.29633594057253 27.402647722287544 23.03254066236432 58 22.095850027029158 27.2625094645723 27.75471088988376 22.886929618514785 59 22.32788383723706 27.42284247113688 27.470845778096052 22.778427913530006 60 22.195450190776196 27.557881296946267 27.540997159680565 22.70567135259697 61 22.416583701581867 27.19161732427413 27.56341734639815 22.828381627745852 62 22.14827170628613 27.5433892838221 27.573431377686177 22.73490763220559 63 22.142391373960024 27.210801180724154 27.669612654940934 22.977194790374885 64 22.134864627102292 27.26889261633604 27.604624854956572 22.9916179016051 65 22.13031693544936 27.280441572331455 27.550238284526074 23.039003207693106 66 22.125507251439515 27.502052294202922 27.50082965084799 22.87161080350957 67 22.087168649095535 27.293446049406434 27.54015801209835 23.079227289399682 68 22.128738523163268 27.243783440751283 27.616602332337752 23.010875703747693 69 22.123460715007305 27.329852565188705 27.400917762922305 23.14576895688168 70 22.05677722855862 27.10076327878015 27.678753369546865 23.163706123114363 71 22.27703749504392 27.191606596929297 27.42486989014239 23.106486017884393 72 22.12309107528572 27.4358548897001 27.276503705773784 23.164550329240388 73 22.225472900983352 27.22588044876833 27.350852065976166 23.19779458427215 74 22.14719461571155 27.4529427861131 27.184747233041644 23.215115365133705 75 22.116541200170005 27.41061603758755 27.3203150926589 23.152527669583545 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 705.0 1 627.0 2 746.0 3 938.0 4 933.0 5 773.5 6 747.5 7 1503.0 8 2125.0 9 3654.0 10 5936.0 11 6271.5 12 5854.0 13 5403.5 14 5221.5 15 5788.5 16 6087.0 17 6675.0 18 7851.0 19 9347.5 20 10256.0 21 11623.0 22 15135.0 23 19850.5 24 22421.0 25 25160.5 26 32183.0 27 41132.0 28 45798.0 29 51190.5 30 60731.5 31 70698.5 32 76517.0 33 81772.0 34 93879.0 35 106865.0 36 112999.0 37 116989.5 38 124352.5 39 131182.0 40 134639.0 41 139620.5 42 147881.0 43 153718.5 44 156277.0 45 159704.5 46 164294.5 47 166414.0 48 167371.0 49 167810.5 50 166631.0 51 161510.0 52 158008.0 53 154202.5 54 143888.0 55 130808.0 56 124237.0 57 116046.5 58 99416.0 59 82495.5 60 74015.0 61 66097.5 62 51291.0 63 37721.0 64 31040.0 65 26342.5 66 18240.0 67 12482.0 68 10129.0 69 8383.5 70 5432.5 71 3494.0 72 2761.0 73 2372.5 74 1770.5 75 1176.0 76 795.0 77 646.0 78 357.5 79 171.5 80 125.0 81 96.5 82 42.0 83 17.5 84 19.0 85 12.5 86 4.0 87 2.5 88 3.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21678631105211374 2 0.005385452872920779 3 2.9110556069842042E-5 4 0.0 5 3.493266728381045E-4 6 0.03775639122258513 7 0.024016208757619686 8 0.007277639017460512 9 0.005152568424362042 10 0.0033768245041016776 11 0.00128086446707305 12 0.021454479823473586 13 9.024272381651034E-4 14 0.0015137489156317864 15 0.0054436739850604625 16 8.733166820952613E-5 17 4.948794531873148E-4 18 0.0 19 0.0011644222427936819 20 0.02718925936923247 21 0.02419087209403874 22 5.8221112139684085E-5 23 0.0 24 0.0 25 2.3288444855873634E-4 26 2.9110556069842042E-5 27 0.0 28 8.733166820952613E-5 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 5.8221112139684085E-5 36 9.315377942349454E-4 37 2.3288444855873634E-4 38 1.1644222427936817E-4 39 0.0 40 2.9110556069842042E-5 41 2.0377389248889431E-4 42 2.3288444855873634E-4 43 0.0 44 0.0 45 0.0 46 2.9110556069842042E-5 47 3.202161167682625E-4 48 0.0 49 2.9110556069842047E-4 50 4.3665834104763065E-4 51 4.0754778497778863E-4 52 5.239900092571568E-4 53 0.0 54 5.8221112139684085E-5 55 0.0 56 2.9110556069842042E-5 57 2.9110556069842042E-5 58 2.9110556069842042E-5 59 0.0 60 8.733166820952613E-5 61 0.0 62 0.0 63 0.0 64 5.8221112139684085E-5 65 2.9110556069842042E-5 66 0.0 67 0.0 68 0.0 69 0.008907830157371666 70 0.0 71 1.7466333641905226E-4 72 0.0 73 0.0 74 0.0 75 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3435180.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.317647526582185 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63395718843877 44.10794456355979 2 12.029805257491267 14.030998856396021 3 5.039184715162693 8.81620194120595 4 2.4781851716957752 5.7808771739422715 5 1.3840982495014214 4.035867692829164 6 0.8909835462480052 3.1176038641245243 7 0.583294373180319 2.381144896155798 8 0.3943922670822735 1.8400023375130952 9 0.289157335737501 1.5176678026738653 >10 1.2305996349875357 11.829506499020244 >50 0.03646850496224184 1.4078805684320124 >100 0.009622087641664905 0.8695952387899013 >500 1.006671498147163E-4 0.04297331403167914 >1k 1.5100072066567876E-4 0.2217352513257511 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3458 0.10066430288951381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.9110556069842046E-5 0.0 2 0.0 0.0 0.0 2.9110556069842046E-5 0.0 3 0.0 0.0 0.0 2.9110556069842046E-5 0.0 4 0.0 0.0 0.0 5.822111213968409E-5 0.0 5 0.0 0.0 0.0 5.822111213968409E-5 0.0 6 0.0 0.0 0.0 5.822111213968409E-5 0.0 7 0.0 0.0 0.0 5.822111213968409E-5 2.9110556069842046E-5 8 0.0 0.0 0.0 5.822111213968409E-5 2.9110556069842046E-5 9 0.0 0.0 0.0 5.822111213968409E-5 2.9110556069842046E-5 10 0.0 0.0 0.0 5.822111213968409E-5 2.9110556069842046E-5 11 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 12 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 13 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 14 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 15 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 16 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 17 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 18 0.0 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 19 2.9110556069842046E-5 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 20 2.9110556069842046E-5 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 21 2.9110556069842046E-5 2.9110556069842046E-5 0.0 5.822111213968409E-5 2.9110556069842046E-5 22 2.9110556069842046E-5 2.9110556069842046E-5 0.0 1.1644222427936818E-4 2.9110556069842046E-5 23 2.9110556069842046E-5 2.9110556069842046E-5 0.0 1.746633364190523E-4 2.9110556069842046E-5 24 2.9110556069842046E-5 2.9110556069842046E-5 0.0 3.202161167682625E-4 2.9110556069842046E-5 25 2.9110556069842046E-5 2.9110556069842046E-5 0.0 3.202161167682625E-4 2.9110556069842046E-5 26 2.9110556069842046E-5 2.9110556069842046E-5 0.0 4.948794531873148E-4 2.9110556069842046E-5 27 2.9110556069842046E-5 2.9110556069842046E-5 0.0 6.40432233536525E-4 2.9110556069842046E-5 28 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.0010188694624444715 2.9110556069842046E-5 29 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.0017757439202603649 2.9110556069842046E-5 30 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.0027946133827048366 2.9110556069842046E-5 31 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.0059385534382477775 2.9110556069842046E-5 32 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.009373599054489139 2.9110556069842046E-5 33 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.01365285079675592 2.9110556069842046E-5 34 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.01863075588469891 2.9110556069842046E-5 35 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.024656640991156213 2.9110556069842046E-5 36 2.9110556069842046E-5 2.9110556069842046E-5 0.0 0.03312781280748025 2.9110556069842046E-5 37 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.04287984909087733 2.9110556069842046E-5 38 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.054262076514185575 2.9110556069842046E-5 39 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.06654673117565892 2.9110556069842046E-5 40 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.0783073958278751 2.9110556069842046E-5 41 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.09108692994253577 2.9110556069842046E-5 42 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.10325514237972974 2.9110556069842046E-5 43 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.11705354595683487 2.9110556069842046E-5 44 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.1332099045755972 2.9110556069842046E-5 45 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.15175332879208658 2.9110556069842046E-5 46 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.17061696912534424 2.9110556069842046E-5 47 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.1894223883464622 2.9110556069842046E-5 48 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.20866446590862778 2.9110556069842046E-5 49 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.22927473960607594 2.9110556069842046E-5 50 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.25032167164457175 2.9110556069842046E-5 51 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.2713977142391374 2.9110556069842046E-5 52 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.29302685739903006 2.9110556069842046E-5 53 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.31538376446066874 2.9110556069842046E-5 54 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.33934175210614875 2.9110556069842046E-5 55 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.36533747867651767 2.9110556069842046E-5 56 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.39456447697063907 2.9110556069842046E-5 57 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.4251305608439732 2.9110556069842046E-5 58 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.45499799137163116 2.9110556069842046E-5 59 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.4883295780716003 2.9110556069842046E-5 60 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.5232040242432711 2.9110556069842046E-5 61 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.5582531337513609 2.9110556069842046E-5 62 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.5940591177172666 2.9110556069842046E-5 63 2.9110556069842046E-5 5.822111213968409E-5 0.0 0.6314370717109439 2.9110556069842046E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3360 0.0 40.134052 1 GTATCAA 8245 0.0 39.75624 1 TATCAAC 11615 0.0 28.16868 2 ATCAACG 11915 0.0 27.546337 3 TCAACGC 12230 0.0 26.723965 4 CAACGCA 12445 0.0 26.26228 5 AACGCAG 12855 0.0 25.293007 6 ACGCAGA 15415 0.0 20.749123 7 CGCAGAG 15570 0.0 20.274576 8 GAGTACT 10095 0.0 19.855877 12 AGAGTAC 14850 0.0 19.538391 11 CAGAGTA 15635 0.0 18.822203 10 AGTACTT 10580 0.0 18.813309 13 CGTTCGT 130 1.18294665E-4 18.574768 40 GCAGAGT 16325 0.0 18.428186 9 GTACATG 10120 0.0 18.24519 1 GTACTTT 11015 0.0 18.229849 14 TACATGG 10210 0.0 17.611216 2 TACTTTT 12220 0.0 16.517406 15 ACTTTTT 13010 0.0 16.33656 16 >>END_MODULE