FastQCFastQC Report
Mon 6 Feb 2017
SRR2079227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4175805
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52650.033.180691
GGTATCA26300.032.2257271
TCAACGC66700.026.2765484
ATCAACG68400.025.6234743
CAACGCA68300.025.3076975
TATCAAC69050.024.8549882
AACGCAG71400.024.4988296
GTACATG119100.020.4190671
ACGCAGA86650.019.8686167
CGCAGAG88200.019.4419168
TACATGG121700.019.408252
ACATGGG126450.017.6256083
AGAGTAC84250.017.24785811
GTGGTAT17750.016.7607121
CAGAGTA95300.015.68259610
GTATAGG8400.015.6493641
CATGGGG92050.015.5919334
GCAGAGT101300.015.3331469
GTATTAC7400.014.9592781
CGTACAC6100.014.1397123
Produced by FastQC (version 0.11.5)