FastQCFastQC Report
Mon 6 Feb 2017
SRR2079227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4175805
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62100.036.418261
GGTATCA29000.036.1308061
TATCAAC88350.025.3150372
TCAACGC87100.025.2820724
ATCAACG88200.025.1232933
CAACGCA88400.024.7540995
AACGCAG94400.023.2212036
GTACATG121150.019.4096871
ACGCAGA115900.018.6401317
TACATGG123000.018.6045252
CGCAGAG118350.017.8136568
ACATGGG128250.017.7352873
AGAGTAC113800.016.67613211
GAGTACT55550.016.33603312
CAGAGTA123600.015.74474110
CATGGGG90700.015.2597444
GTACTTT59750.015.24583914
GCAGAGT130950.015.1508599
AGTACTT62950.014.412909513
TAGTCGG3152.2488166E-814.23600340
Produced by FastQC (version 0.11.5)