FastQCFastQC Report
Mon 6 Feb 2017
SRR2079226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726662
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41200.033.424231
GGTATCA20800.031.7720761
TCAACGC50650.026.6292674
ATCAACG51350.026.199083
CAACGCA52100.025.756075
TATCAAC54300.024.8592912
AACGCAG55700.023.9056116
GTACATG85350.020.2694531
ACGCAGA65700.019.5316737
TATACCG2505.0931703E-1119.3177135
TACATGG86300.019.0417982
CGCAGAG68500.018.5321148
ACATGGG91500.017.5681783
AGAGTAC65850.016.8749611
CAGAGTA73750.015.48847510
CATGGGG70350.015.4457254
GCAGAGT77300.015.1789629
GTGTAGC14700.014.8285541
CGAATCG2901.2648525E-714.27302437
CGTGCCG5250.013.80392710
Produced by FastQC (version 0.11.5)