FastQCFastQC Report
Mon 6 Feb 2017
SRR2079226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726662
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55650.039.088231
GGTATCA24050.036.9554561
TATCAAC80750.026.3678872
ATCAACG82100.026.2705753
TCAACGC81600.026.2623844
CAACGCA84300.025.5031135
AACGCAG89900.023.8418756
ACGCAGA105750.019.807227
GTACATG92750.019.649761
GAGTACT60050.019.36279312
TACATGG92950.019.3428752
CGCAGAG108100.019.24618
AGTACTT63550.018.67292813
AGAGTAC102550.018.67275611
GTACTTT65550.018.1604514
ACATGGG97400.017.9985473
CAGAGTA110750.017.6017510
GCAGAGT117000.016.8973989
TACTTTT76500.015.29059315
ACTTTTT80450.015.2689816
Produced by FastQC (version 0.11.5)