FastQCFastQC Report
Mon 6 Feb 2017
SRR2079225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3823996
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47900.031.760061
GGTATCA25950.027.5801811
TCAACGC56900.026.1923434
ATCAACG57800.025.5457573
CAACGCA59050.025.1802625
TATCAAC59950.024.704932
AACGCAG62600.023.5869796
ACGCAGA72250.019.958077
GTACATG88200.019.9532361
TACATGG86950.019.4157752
CGCAGAG74300.018.8514948
AGAGTAC68150.017.72317311
ACATGGG92450.017.5758723
CATGGGG68550.016.3057374
CAGAGTA78350.016.0765610
GTATATA14900.015.5472161
GCAGAGT84850.015.4552739
GAGTACT30650.014.86179812
GTATAGC11000.014.4587011
GTACTTT33750.014.21170514
Produced by FastQC (version 0.11.5)