FastQCFastQC Report
Mon 6 Feb 2017
SRR2079225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3823996
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63350.038.8622671
GGTATCA29400.032.4605941
TATCAAC92100.026.9058272
ATCAACG93050.026.705313
TCAACGC94200.026.2693774
CAACGCA97550.025.1903535
AACGCAG101600.024.3281356
ACGCAGA120600.019.945217
CGCAGAG123700.019.2483188
GAGTACT78200.018.75382612
GTACATG91200.018.6157881
AGAGTAC118850.018.29148911
TACATGG89850.018.1303182
GTACTTT83300.017.5621414
AGTACTT83800.017.45506913
ACATGGG95250.017.1749273
CAGAGTA130050.016.98154810
GCAGAGT136000.016.5938249
CTATACG2804.7184585E-916.023854
CATGGGG66950.015.4650814
Produced by FastQC (version 0.11.5)