FastQCFastQC Report
Mon 6 Feb 2017
SRR2079224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220121
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.032.9683571
GTATCAA41250.032.5453951
TCAACGC51450.025.6107334
CAACGCA54000.024.7846035
ATCAACG53600.024.5834353
TATCAAC54700.024.1787972
AACGCAG57850.023.1947776
GTACATG86250.020.9006791
TACATGG87500.020.0483632
ACGCAGA66500.019.1909777
CGCAGAG67700.018.54578
ACATGGG92850.018.500843
AGAGTAC61750.017.49230611
TATACCG2057.778708E-716.8263595
GTGTAGC12950.016.5654661
CAGAGTA69000.016.20449810
CGTACAC4200.015.6044443
CATGGGG65000.015.548854
GCAGAGT75400.015.3782749
ATACCGA2503.6820347E-715.1773766
Produced by FastQC (version 0.11.5)