FastQCFastQC Report
Mon 6 Feb 2017
SRR2079224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220121
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63750.040.6277351
GGTATCA26800.039.7408261
TATCAAC93450.027.5874372
TCAACGC95700.026.722454
ATCAACG96700.026.5531793
CAACGCA99000.025.7968435
AACGCAG103700.024.5981946
CGAATCG1502.8952854E-822.99692237
ACGCAGA122300.020.6547977
CGCAGAG125850.019.9867978
GAGTACT72250.019.91408312
GTACATG94050.019.0087131
AGAGTAC122750.018.80471411
GTATAGG6800.018.3068891
CAGAGTA129800.018.15549510
AGTACTT78900.018.10164313
TACATGG97000.018.0387422
GCAGAGT134600.017.969469
GTACTTT80650.017.5421214
ACATGGG103300.016.6045113
Produced by FastQC (version 0.11.5)