FastQCFastQC Report
Mon 6 Feb 2017
SRR2079223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029668
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49700.032.016781
GGTATCA25900.030.1845651
TCAACGC58900.026.531764
ATCAACG59300.026.2946193
CAACGCA61600.025.2008365
TATCAAC63800.024.4086382
AACGCAG65050.023.811256
ACGCAGA74800.020.2460677
CGCAGAG75150.019.830948
GTACATG85150.019.0936721
AGAGTAC69950.017.90713511
TACATGG85600.017.8293762
ACATGGG87700.017.0322283
CAGAGTA78550.016.51767210
GCAGAGT84300.016.168769
GTCTTAG12700.014.436051
CATGGGG64200.014.2394764
GAGTACT32600.013.65393212
GAGTACA62300.013.2149391
GTATTAG11450.012.9908991
Produced by FastQC (version 0.11.5)