FastQCFastQC Report
Mon 6 Feb 2017
SRR2079223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029668
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28950.039.6612471
GTATCAA69400.039.2186051
ATCAACG97800.028.1960893
TCAACGC98250.027.891314
TATCAAC96400.027.8895442
CAACGCA101400.026.8546815
AACGCAG106350.025.4138186
ACGCAGA125050.021.0556147
CGCAGAG126350.020.809088
GAGTACT85750.019.11292812
TACATGG88200.018.821642
GTACATG88650.018.8037821
AGAGTAC126950.018.7535811
CAGAGTA132800.018.26522410
GTACTTT92300.018.0565514
AGTACTT91450.017.918313
GCAGAGT139500.017.6847769
ACATGGG92650.017.358873
GTCTAGG8700.016.6957871
CATGGGG62500.015.6274234
Produced by FastQC (version 0.11.5)