FastQCFastQC Report
Mon 6 Feb 2017
SRR2079222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028985
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40350.035.073631
GGTATCA18850.033.5923771
ATCAACG49800.027.9165693
TCAACGC49750.027.6672614
CAACGCA51600.026.9427365
TATCAAC53150.026.3796142
AACGCAG55000.024.8381276
ACGCAGA63400.020.7310877
CGCAGAG64900.019.827038
GTACATG69350.018.760411
AGAGTAC63250.018.1140111
TACATGG67650.018.0199092
ACATGGG70750.017.212133
CAGAGTA69500.016.93193410
GCAGAGT73400.016.6429339
CGCACGA1704.9768267E-516.23085428
GAGTACT34450.016.22657612
CGTATAC1756.3662E-515.7702413
GTGTACG2654.0296072E-815.6688221
TATCGTA1605.765218E-415.0926137
Produced by FastQC (version 0.11.5)