FastQCFastQC Report
Mon 6 Feb 2017
SRR2079222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028985
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59350.041.5536961
GGTATCA21900.039.8013041
ATCAACG86000.028.853253
TCAACGC86000.028.732864
TATCAAC85400.028.6922632
CAACGCA88150.028.0320575
AACGCAG91550.026.9604266
ACGCAGA108350.022.6174647
CGCAGAG110150.022.0853528
GAGTACT80200.021.1674712
AGAGTAC108500.020.63921511
AGTACTT86100.019.8720213
CAGAGTA114800.019.7169710
GCAGAGT118500.019.5381039
GTACTTT89900.018.76995514
GTACATG63250.018.6481061
TACATGG62800.018.2449532
TCGCGTC5300.018.23040217
ACATGGG64400.018.1667863
GTCGCGT5500.017.56747816
Produced by FastQC (version 0.11.5)