FastQCFastQC Report
Mon 6 Feb 2017
SRR2079221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316477
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.033.9860841
GGTATCA14200.031.1728021
TCAACGC36000.026.83234
CAACGCA35800.026.6931085
ATCAACG36200.026.5887573
TATCAAC37300.026.1915662
AACGCAG37950.024.9081336
CGCAGAG43700.020.6829688
ACGCAGA43550.020.6745457
GTACATG46950.018.5617621
TACATGG47550.018.0046652
AGAGTAC43200.017.97659911
GAGTACT23500.017.47595812
ACATGGG49000.017.3197423
GTATTAG5900.016.9980951
CAGAGTA48100.016.79111710
GTGTACG3351.0913936E-1116.5169331
GCAGAGT51550.016.2692539
GTACTTT26800.015.19430814
AGTACTT28000.015.03708613
Produced by FastQC (version 0.11.5)