FastQCFastQC Report
Mon 6 Feb 2017
SRR2079221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316477
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.041.4286651
GTATCAA43750.039.9245721
TATCAAC62800.027.9172952
TCAACGC63350.027.8928324
ATCAACG63750.027.7719553
CAACGCA65050.026.9516725
AACGCAG67050.026.1528246
ACGCAGA79650.021.7085387
CCGCGTA800.002898126821.565310
CGCAGAG81900.021.023338
AGAGTAC79600.019.98312211
GAGTACT59300.019.78334412
TCGTATC1751.4723446E-719.71684618
AGTACTT61200.019.33453813
GTACATG50100.019.3306871
GTACTTT61400.019.21910714
CAGAGTA84300.019.15551410
GCAGAGT87350.018.802679
TACATGG49800.018.5033862
CGTATCC1702.4314231E-618.26707819
Produced by FastQC (version 0.11.5)