FastQCFastQC Report
Mon 6 Feb 2017
SRR2079220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745080
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49150.036.029361
GGTATCA23500.029.2883821
TCAACGC63400.027.044164
ATCAACG64950.026.5581153
CAACGCA65600.026.0849535
TATCAAC67450.025.5427362
AACGCAG69100.025.1132056
ACGCAGA79250.021.591227
CGCAGAG81350.020.567938
GTACATG96800.019.2228181
TACATGG95700.018.616012
AGAGTAC79450.018.5055511
CAGAGTA85550.017.54912810
ACATGGG101750.017.0545393
GCAGAGT92150.016.741219
GAGTACT36800.016.6921412
CATGGGG79000.014.9786774
GTATAAA16550.014.83783151
GAGTACA72550.014.7786381
GTACTTT44650.013.91111414
Produced by FastQC (version 0.11.5)