FastQCFastQC Report
Mon 6 Feb 2017
SRR2079220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745080
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.041.7887151
GTATCAA75600.039.8488581
TATCAAC105950.028.1818332
ATCAACG110050.027.351463
TCAACGC110250.027.0200584
CAACGCA113250.026.2738155
AACGCAG117900.025.1547786
ACGCAGA139600.021.0869267
CGAATCG2850.020.57551437
CGCAGAG143800.020.3003318
GAGTACT94450.020.09254512
AGAGTAC138550.019.27563311
AGTACTT99300.019.00313813
GTACATG92800.018.933691
GTACTTT100800.018.69037214
CAGAGTA148850.018.26634410
TACATGG95100.018.1849142
GCAGAGT153750.017.9312959
TATTCCG2802.5283953E-1017.2593365
TATACCG2005.963375E-717.2593355
Produced by FastQC (version 0.11.5)