FastQCFastQC Report
Mon 6 Feb 2017
SRR2079219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948302
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42100.030.7406231
GGTATCA23250.029.9154971
TCAACGC49900.024.0590864
ATCAACG51200.023.8524913
CAACGCA51850.022.888415
TATCAAC54650.022.6212832
AACGCAG56450.020.901056
ACGCAGA65350.017.5257427
GTACATG84100.017.4458871
CGCAGAG68550.016.808488
TACATGG85900.016.6027972
GTGTAGC20200.015.9314281
AGAGTAC65800.015.47018911
ACATGGG91500.015.2321323
GTACTAA7750.014.73451
GTATTAG8600.014.082921
TATACTG13000.014.0649595
CAGAGTA75850.013.73886610
GTATAAT13100.013.7358391
GTACTAT7950.013.4932851
Produced by FastQC (version 0.11.5)