FastQCFastQC Report
Mon 6 Feb 2017
SRR2079219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948302
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62800.038.600341
GGTATCA25800.033.2402841
TATCAAC86400.027.7605512
ATCAACG87950.027.6244623
TCAACGC89100.027.1129864
CAACGCA90650.026.649395
AACGCAG95450.025.2762896
ACGCAGA112700.021.0655127
CGCAGAG117000.020.0523178
GAGTACT76450.019.496112
AGAGTAC112550.019.31241411
GTACATG84750.018.8510281
AGTACTT81950.018.18487413
CAGAGTA122700.018.1647510
GTACTTT81800.018.09651814
TACATGG86550.018.0230542
GCAGAGT128200.017.4661889
CGTACAC4850.017.0775893
ACATGGG95300.016.2596233
TACTTTT94250.015.7426615
Produced by FastQC (version 0.11.5)