FastQCFastQC Report
Mon 6 Feb 2017
SRR2079218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3990228
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42500.031.2682151
ATCAACG49150.026.1122343
GGTATCA24050.026.0449961
TCAACGC49850.025.1226864
CAACGCA51650.024.313965
TATCAAC53800.024.2014472
AACGCAG53950.023.021616
GTACATG71750.019.871751
ACGCAGA61500.019.2965987
TACATGG71050.018.9575772
CGCAGAG63450.018.4863058
ACATGGG74500.016.8102823
AGAGTAC63650.016.69903211
GTATAGC11850.016.0622021
GAGTACT34850.015.54564212
CAGAGTA70800.015.35381510
GCAGAGT77250.014.0718459
GTATAGG10100.013.7056421
GTACTTT39200.013.55544414
CATGGGG49400.013.5487564
Produced by FastQC (version 0.11.5)