FastQCFastQC Report
Mon 6 Feb 2017
SRR2079218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3990228
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59300.038.9527781
GGTATCA26300.033.9219361
ATCAACG83400.027.3524633
TATCAAC84400.027.273722
TCAACGC84350.026.9625724
CAACGCA87200.025.8043025
AACGCAG89850.025.0482676
ACGCAGA109600.020.1848167
TCGCGCT1551.0070653E-620.02951461
CGCAGAG110100.019.7742338
GAGTACT75100.018.33118612
AGAGTAC106250.017.990311
GCGATCG1954.606718E-717.68988424
GTACATG75500.017.6789721
CGCGATC1804.206924E-617.24763723
CAGAGTA113900.017.14550610
TACATGG74950.016.9908752
GTACTTT82200.016.91679414
CGATCGT2057.7769255E-716.82696325
AGTACTT83700.016.81511313
Produced by FastQC (version 0.11.5)