FastQCFastQC Report
Mon 6 Feb 2017
SRR2079217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437479
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38250.033.358251
GGTATCA19900.029.8870961
ATCAACG48150.025.8669223
TCAACGC47850.025.7406864
CAACGCA50050.024.6095855
TATCAAC51350.024.3437612
AACGCAG52850.023.371056
GTACATG76050.019.3240451
ACGCAGA64550.018.8668637
CGCAGAG65350.018.530588
TACATGG77800.018.375552
ACATGGG82150.017.177093
GAGTACT28050.016.73271212
AGAGTAC63450.016.37246711
GTATAGC8950.016.2268661
GTGTAGC15500.015.6161761
CAGAGTA69100.015.5831710
GTGTACG3603.45608E-1115.36831
GCAGAGT73650.015.1827889
CATGGGG59400.014.404534
Produced by FastQC (version 0.11.5)