FastQCFastQC Report
Mon 6 Feb 2017
SRR2079217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437479
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63050.039.6545331
GGTATCA26350.038.058971
TATCAAC89750.027.6486362
TCAACGC92500.027.3490034
ATCAACG93100.027.246893
CAACGCA96150.026.203115
AACGCAG100100.025.2432216
ACGCAGA118900.020.8970817
CGCAGAG121800.020.1138658
GAGTACT83250.019.43896312
AGAGTAC118350.018.89257811
GTACATG83200.018.7453211
AGTACTT86050.018.68178713
GTATAGG7250.018.6022831
CAGAGTA125150.018.36233310
TACATGG83700.018.349062
GTACTTT88600.017.95125414
GCAGAGT132900.017.4732849
TATACTG10850.017.1768425
ACATGGG88100.017.1584263
Produced by FastQC (version 0.11.5)