FastQCFastQC Report
Mon 6 Feb 2017
SRR2079216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914857
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37200.035.80391
GGTATCA18100.029.8166331
TCAACGC47750.028.1798974
CAACGCA47650.027.9498845
ATCAACG48000.027.6737273
TATCAAC48050.027.1670072
AACGCAG51050.025.9527656
ACGCAGA59250.021.777557
CGCAGAG62300.020.3791288
AGAGTAC58200.019.09185611
GTACATG73200.018.7625561
GAGTACT25300.017.86667312
TACATGG72200.017.8408282
CAGAGTA65400.017.41211110
GCAGAGT70850.016.6577539
ACATGGG77300.016.4700533
CTATACT5750.016.2010844
AGTACTT28200.015.66196613
GTACTTT28350.015.5788314
GTGGTAT11650.015.4415181
Produced by FastQC (version 0.11.5)