FastQCFastQC Report
Mon 6 Feb 2017
SRR2079216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914857
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50200.039.13971
GGTATCA22650.036.958991
TATCAAC71900.027.2199172
TCAACGC75050.026.445384
ATCAACG75700.026.2183043
CAACGCA76150.025.8367315
AACGCAG79850.024.6001116
ACGCAGA93700.020.6624287
CGTGCCG3350.020.59759710
CGCAGAG96550.019.725738
GTACATG72800.019.149021
AGAGTAC94000.018.49839811
TACATGG72400.018.307342
GCACCGT6050.017.6891126
GAGTACT58550.017.61877412
CAGAGTA100500.017.5422910
GTATACG1200.001451455617.2958891
GCAGAGT105600.017.0874259
AGTACTT61700.016.8285113
ACATGGG78800.016.8204523
Produced by FastQC (version 0.11.5)