FastQCFastQC Report
Mon 6 Feb 2017
SRR2079215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702123
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41400.035.4282231
GGTATCA21300.029.8828561
TCAACGC50500.027.6668914
ATCAACG51200.027.4233933
CAACGCA52750.026.3563445
TATCAAC54400.025.7684252
AACGCAG55100.025.044426
ACGCAGA63250.021.0528937
CGCAGAG66900.019.6472348
GTATAGC10450.019.1997491
GTACATG75600.018.7148081
AGAGTAC63450.018.11156811
TACATGG77350.017.2300992
CAGAGTA69800.017.05717810
ACATGGG78850.016.7131523
TCTATAC9900.016.0294783
GTATAGG9500.016.021861
CTATACG1958.99754E-615.9222564
GCAGAGT77000.015.8655829
CAACGCG1808.09574E-515.3327525
Produced by FastQC (version 0.11.5)