FastQCFastQC Report
Mon 6 Feb 2017
SRR2079215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702123
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62600.039.8320961
GGTATCA26250.035.1766241
ATCAACG88300.028.574013
TATCAAC87500.028.5591352
TCAACGC89450.028.0137214
CAACGCA90900.027.4529465
AACGCAG95150.026.2679676
ACGCAGA114700.021.2713097
CGCAGAG117150.020.7650158
AGAGTAC114050.019.45312311
GAGTACT81200.019.20684812
GTACATG77450.018.6203081
CAGAGTA122600.018.4342110
GCAGAGT128450.017.9172469
GTACTTT87650.017.83161214
AGTACTT87750.017.65163413
TACATGG78850.017.2905942
ACATGGG81700.016.6029423
GACCGTA2109.960822E-716.4330797
TACTTTT102600.015.23354615
Produced by FastQC (version 0.11.5)