FastQCFastQC Report
Mon 6 Feb 2017
SRR2079214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749857
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31950.031.4002321
GGTATCA17800.029.346931
TCAACGC38400.026.0513554
ATCAACG39200.025.7836933
CAACGCA40950.024.2610745
TATCAAC41650.023.7908442
AACGCAG42900.023.15836
ACGCAGA48800.020.3587937
CGCAGAG49250.019.892968
GCGCTAA950.00786434518.14698855
GTACATG53750.018.0212651
AGAGTAC48700.017.57357611
GTATAGG6800.017.2971951
TACATGG54850.016.8694572
TAGCCCT15650.016.3110124
GTACTAG5650.015.9195441
CAGAGTA55050.015.67184410
CGTGCGC2654.1640305E-815.62689810
GTATAGC9750.015.6118291
ACATGGG57250.015.6058653
Produced by FastQC (version 0.11.5)