FastQCFastQC Report
Mon 6 Feb 2017
SRR2079214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749857
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47150.040.193161
GGTATCA20200.035.780651
TCAACGC64750.028.8875874
TATCAAC65400.028.864322
ATCAACG65450.028.7895433
CAACGCA67450.027.9870535
AACGCAG71450.026.3289156
CCGCGTA809.5585434E-525.87819910
TACCGTA1803.4197E-1023.0053547
ACGCAGA82200.022.2916857
CGCAGAG82900.021.7684678
GAGTACT56850.020.81787912
AGAGTAC78750.020.41776311
CAGAGTA85850.019.1712710
GCAGAGT91450.018.6387189
GTACATG53000.018.4656181
AGTACTT64200.018.16216313
GTACTTT64150.018.12452114
TACATGG50600.018.0737022
CGCGTAC1150.00110983718.00222611
Produced by FastQC (version 0.11.5)