FastQCFastQC Report
Mon 6 Feb 2017
SRR2079213_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079213_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761854
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44250.032.372961
GGTATCA22700.030.4859331
ATCAACG51300.026.7655963
TCAACGC51500.026.5946644
CAACGCA53400.025.3906655
TATCAAC56800.024.3153842
AACGCAG57550.023.4997656
GTACATG78250.020.208181
ACGCAGA65500.019.9095637
TACATGG79000.019.3618162
CGCAGAG66800.019.2122278
ACATGGG82250.017.8264263
AGAGTAC63400.017.58155811
GCAGAGT73850.016.0278829
CAGAGTA70400.015.98045110
GAGTACT35050.015.94910812
GTATAGC11750.015.60751
TAGCCCT22900.014.9145774
CATGGGG55700.014.5553924
GAGTACA57500.014.3822031
Produced by FastQC (version 0.11.5)