FastQCFastQC Report
Mon 6 Feb 2017
SRR2079213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761854
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68300.039.4493681
GGTATCA27850.035.270891
ATCAACG94550.028.7299253
TCAACGC94200.028.6168254
TATCAAC93550.028.4835972
CAACGCA97700.027.3796835
AACGCAG102600.026.2122216
ACGCAGA121950.021.4505987
CGCAGAG124200.020.8633338
GAGTACT87600.019.61458212
AGAGTAC121850.019.50956211
CAGAGTA128750.018.65158810
GCAGAGT135250.018.0868439
AGTACTT95200.018.008413
GTACATG80200.017.8976921
GTACTTT95100.017.88795314
TACATGG79500.017.0626582
ACATGGG85300.016.3071213
TACTTTT110000.015.6535815
TCGTATG5800.015.46284940
Produced by FastQC (version 0.11.5)