FastQCFastQC Report
Mon 6 Feb 2017
SRR2079212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3356620
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61430.18301148178822743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37900.037.8907741
GTATCAA86950.037.648371
ATCAACG113100.028.06133
TCAACGC114350.027.6338834
TATCAAC116650.027.3821182
CAACGCA116350.027.0110245
AACGCAG121950.025.7989546
TAGTCGG5850.022.9960140
GTACATG192250.022.2112711
ACGCAGA142600.021.4082167
TTAGTCG6350.021.1906739
CGCAGAG143850.020.98278
TACATGG196300.020.9508442
ACATGGG194450.020.6681023
AGAGTAC139500.019.91524111
CAGAGTA144350.019.46128510
GCAGAGT149000.019.0852729
GAGTACT49400.019.07129512
GTGGTAT20250.018.9680441
CATGGGA96750.018.5410774
Produced by FastQC (version 0.11.5)