FastQCFastQC Report
Mon 6 Feb 2017
SRR2079212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3356620
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66790.19897992623532004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.1316502910666087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33860.10087528525719325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.046.4373971
GTATCAA152050.043.0207371
TATCAAC218900.029.8013632
ATCAACG226500.029.1366623
TCAACGC230000.028.5732214
CAACGCA234850.027.9390535
AACGCAG241700.027.0818866
CTTAACG1502.8894647E-823.0013921
ACGCAGA281350.022.9983567
CGCAGAG284150.022.6219448
GAGTACT187900.022.4209312
AGTACTT201000.021.64146813
AGAGTAC281000.021.3161211
GTACATG183850.021.2988171
GTACTTT198150.021.29500614
CAGAGTA286950.021.07853510
GCAGAGT291150.020.9166769
TACATGG190950.020.3534872
TTAACGC1701.088174E-720.29111322
ACATGGG192750.019.9843443
Produced by FastQC (version 0.11.5)