FastQCFastQC Report
Mon 6 Feb 2017
SRR2079211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629010
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53850.037.393341
GGTATCA25100.033.9092641
TCAACGC70800.027.6800464
ATCAACG70800.027.6313133
TATCAAC73850.026.7970642
CAACGCA73450.026.5404555
AACGCAG75200.025.647546
ACGCAGA86950.021.7048857
CGCAGAG90850.020.3174218
GTACATG107550.019.7200051
TACATGG105100.019.4536932
AGAGTAC87100.018.94083811
ACATGGG107700.018.5487483
CAGAGTA91650.018.30177710
GCAGAGT96850.017.924459
GAGTACT41500.016.96358512
CCGATCG1503.5216598E-416.10365114
GTGGTAT16150.016.0674041
GTATATA20350.015.4715561
GTACTTT44950.015.27710214
Produced by FastQC (version 0.11.5)