FastQCFastQC Report
Mon 6 Feb 2017
SRR2079211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629010
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92000.041.9090921
GGTATCA36750.041.872021
TATCAAC136700.028.3289552
ATCAACG140500.027.9312483
TCAACGC142700.027.524824
CAACGCA147600.026.5409055
AACGCAG152650.025.7597066
ACGCAGA178650.021.6364157
CGCAGAG181700.021.1358998
GAGTACT128300.020.22393812
AGAGTAC177400.019.91685911
CGTGCCG1952.1549567E-819.4640210
CAGAGTA184350.019.37187810
GCAGAGT188300.019.18549
AGTACTT136050.019.11787413
TACATGG107700.018.9078662
GTACATG110350.018.8331681
CGCGAAT1301.1832595E-418.57417528
GTACTTT139000.018.54275714
ACATGGG111750.018.0681863