FastQCFastQC Report
Mon 6 Feb 2017
SRR2079210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3781390
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44850.029.2387491
GGTATCA23950.027.4492261
ATCAACG50700.025.2447153
TCAACGC51100.024.7095414
CAACGCA52650.024.0479455
TATCAAC54450.023.7184542
AACGCAG56350.022.7747466
GTACATG85550.020.0605891
ACGCAGA64800.019.1660257
TACATGG87400.018.846022
CGCAGAG66750.018.5034858
ACATGGG92500.016.8578133
AGAGTAC65600.016.3618911
GTATAGC10200.015.9433551
CAGAGTA72250.015.23806310
TAGCCCT21800.015.192154
GTGGTAT15500.015.1795621
TAAGGCG9350.015.12563241
GGGTATA7250.014.7946841
GAGTACA62800.014.7658061
Produced by FastQC (version 0.11.5)