FastQCFastQC Report
Mon 6 Feb 2017
SRR2079210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3781390
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.038.2186361
GTATCAA68000.036.4692231
ATCAACG92500.027.276513
TCAACGC94500.026.6627064
TATCAAC94050.026.2397942
CAACGCA98000.025.6400265
AACGCAG102700.024.3056626
CGCGATC800.002903590421.55873123
ACGCAGA124100.019.5795447
CGCAGAG126100.019.2373058
GAGTACT84050.018.31029912
GTACATG89300.018.2812881
TCGATTG1150.001111475417.9985266
AGAGTAC125500.017.98176411
TACATGG89150.017.7319832
CAGAGTA131700.017.3972510
AGTACTT91400.016.79522113
GCAGAGT139150.016.713799
GTACTTT93200.016.51113714
ACATGGG92900.016.421763
Produced by FastQC (version 0.11.5)