FastQCFastQC Report
Mon 6 Feb 2017
SRR2079209_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079209_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583228
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43500.035.0565571
GGTATCA22300.031.6333541
ATCAACG56000.026.6751063
TCAACGC55700.026.5710284
CAACGCA57150.025.77655
TATCAAC58900.025.3264922
AACGCAG60100.024.5112676
ACGCAGA70300.020.7568287
CGCAGAG72800.019.9492538
GTACATG88950.019.282161
CGCGTTA900.005741419319.16396142
AATCGCG950.00783200418.16014519
TACATGG91300.018.1163772
AGAGTAC69800.018.09250311
ACATGGG93450.017.2033443
CAGAGTA76000.017.0705410
GCAGAGT81550.016.1203339
ACCGTAT3058.62201E-1015.8344938
GAGTACT28900.015.7590812
TATACCG2808.214374E-814.7854835
Produced by FastQC (version 0.11.5)