FastQCFastQC Report
Mon 6 Feb 2017
SRR2079209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583228
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61350.038.553651
GGTATCA27250.036.673751
TATCAAC88000.026.7466872
ATCAACG89450.026.6217773
TCAACGC90850.026.0595844
CAACGCA92700.025.4278285
AACGCAG97450.024.3341376
GTACATG87650.020.5546361
ACGCAGA117100.019.8614377
CGCAGAG119500.019.4026268
TACATGG89850.019.3589022
GAGTACT74650.018.71855212
AGAGTAC115050.018.50314311
AGTACTT79800.017.5940513
CAGAGTA123050.017.44037410
GTACTTT79700.017.36078814
ACATGGG97450.017.2824883
GCAGAGT130150.016.8335849
CATGGGG68250.015.1700444
TACTTTT92500.014.92112815
Produced by FastQC (version 0.11.5)