FastQCFastQC Report
Mon 6 Feb 2017
SRR2079208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258138
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.034.3313261
GTATCAA40700.032.291841
TCAACGC49750.026.2128314
ATCAACG50750.025.900263
TATCAAC52350.025.0627692
CAACGCA53800.024.6892015
AACGCAG55100.023.9810986
ACGCAGA64800.019.6982947
CGCAGAG65000.019.1603058
GTACATG81750.018.8267731
TACATGG83000.018.2204112
AGAGTAC62050.017.2428311
ACATGGG86850.016.7235133
CAGAGTA66200.016.52684410
GCAGAGT72700.015.6668449
GTGTAGC15900.015.44418051
TATACCG1808.0911355E-515.3336685
GAGTACA62400.014.9097831
CATGGGG58650.014.8822194
GTATATA12650.013.9438691
Produced by FastQC (version 0.11.5)