FastQCFastQC Report
Mon 6 Feb 2017
SRR2079208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258138
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61250.038.0621071
GGTATCA26200.038.0215871
TATCAAC89050.026.1234442
TCAACGC90000.025.9243954
ATCAACG90800.025.8860443
CAACGCA92950.025.0273535
AACGCAG96500.023.930036
GTACATG88650.021.1865431
TACATGG89700.020.3933142
TCGCACG850.0041233220.29280913
ACGCAGA117450.019.3622937
ACATGGG92200.018.9419213
CGCAGAG119750.018.9004758
AGAGTAC114500.018.01883711
GAGTACT72550.017.88057512
CAGAGTA120400.017.4224110
GCAGAGT125850.016.9968939
AGTACTT78200.016.7195113
CGAACGT1250.001943276116.55791745
GTACTTT79250.016.45673214
Produced by FastQC (version 0.11.5)