FastQCFastQC Report
Mon 6 Feb 2017
SRR2079207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612297
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41700.033.6034361
GGTATCA22050.029.6563631
ATCAACG52600.026.5613963
TCAACGC52800.026.3954494
CAACGCA54950.025.174355
TATCAAC54700.025.1238732
AACGCAG58300.023.9053126
ACGCAGA67200.020.2764267
GTACATG84900.020.1726151
ACGTTCG1409.303609E-619.72624626
CGCAGAG68050.019.72018
TACATGG83600.019.412542
AGAGTAC63250.018.00742311
ACATGGG86550.017.7766283
CAGAGTA70250.016.45874410
GCAGAGT75000.016.1528849
GTGTAGC17350.015.7540531
GAGTACT30750.015.15132912
TACACCG4101.8189894E-1215.1450345
CATGGGG65200.014.7088534
Produced by FastQC (version 0.11.5)