FastQCFastQC Report
Mon 6 Feb 2017
SRR2079207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612297
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51400.038.222951
GGTATCA22400.033.6625861
ATCAACG71650.027.8426973
TCAACGC72650.027.1744084
TATCAAC72250.027.0862
CAACGCA75850.025.8459475
AACGCAG78800.024.8383466
ACGCAGA96900.019.8723837
GTACATG81000.019.5150741
CGCAGAG99700.018.9996038
GAGTACT61400.018.99344312
TACATGG79000.018.9173562
AGAGTAC93950.018.6561611
TTAGTCG1301.1832935E-418.574139
ACATGGG84950.017.7548793
CAGAGTA102950.017.32684710
GTACTTT68250.017.29031414
AGTACTT68850.016.93565413
GCAGAGT108700.016.727949
GTATAGC11000.015.4078621