FastQCFastQC Report
Mon 6 Feb 2017
SRR2079206_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079206_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115950
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31440.14858574162905552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56000.038.563341
GGTATCA25150.038.2547071
TATCAAC73500.029.0771922
ATCAACG75800.028.5376853
TCAACGC76300.028.2150294
CAACGCA77700.027.751055
AACGCAG81350.026.0818256
CGCAGAG94500.021.8672878
ACGCAGA94900.021.8473057
GAGTACT36500.021.18118112
GTACTTT36950.020.73444714
AGAGTAC93250.020.72787511
GTACATG115850.020.4631561
TACATGG114950.020.2441772
GCAGAGT98200.020.1399679
CAGAGTA97650.020.04132510
AGTACTT40550.019.31964913
ACATGGG120700.018.8650113
GTGGTAT13300.018.4750161
TGGTATC11800.018.1409022
Produced by FastQC (version 0.11.5)