FastQCFastQC Report
Mon 6 Feb 2017
SRR2079206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115950
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37440.17694179919185235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25680.12136392636877052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.047.822671
GTATCAA88500.044.535381
TATCAAC128550.030.773592
ATCAACG134850.029.7710673
TCAACGC137350.029.1035234
CAACGCA139450.028.442465
AACGCAG144350.027.4556566
TGGTCGG2800.023.41072310
ACGCAGA170500.022.9914077
CGCAGAG170250.022.8784228
CGTCCGA2005.2750693E-1122.42500711
TATATCG1854.8567017E-1022.3762766
TAGTCGG2350.022.01764140
GAGTACT110750.021.74357612
AGAGTAC168400.021.69567311
GCAGAGT174500.021.3326729
CAGAGTA173650.021.19868310
AGTACTT115950.021.09274713
TACATGG116950.020.7796442
GTACATG117950.020.6831131
Produced by FastQC (version 0.11.5)