FastQCFastQC Report
Mon 6 Feb 2017
SRR2079205_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079205_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601219
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43600.034.1258351
TCAACGC51700.028.294474
ATCAACG52400.028.1140143
CAACGCA55400.026.3428575
TATCAAC56350.026.1654662
GGTATCA22350.025.390291
AACGCAG59150.025.0227386
ACGCAGA66950.021.2829487
CGCAGAG67950.020.7675028
AGAGTAC63850.019.35296811
GTACATG69350.019.1595861
TACATGG68300.018.6046542
CAGAGTA72900.017.56595610
GAGTACT37000.017.53635412
GTATAGC12450.017.509451
ACATGGG74750.017.2156683
GCAGAGT77450.017.2029699
TAGCCCT20300.016.6554474
CGCGATC1300.002506739715.93862123
GTATAGG10450.015.8937461
Produced by FastQC (version 0.11.5)