FastQCFastQC Report
Mon 6 Feb 2017
SRR2079205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601219
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63650.040.4281431
GGTATCA27050.029.919741
ATCAACG89100.029.3246973
TCAACGC90150.028.9065724
TATCAAC90400.028.4066412
CAACGCA94400.027.3857845
AACGCAG98050.026.5097086
ACGCAGA113550.022.4284937
CGCAGAG116850.021.7320948
CGCGTAA800.002901897321.56086565
AGAGTAC110900.021.03049711
GAGTACT82350.020.65464412
CAGAGTA121800.019.46004510
GCAGAGT124550.019.3073849
AGTACTT90750.018.77900313
GTACTTT91850.018.70849414
GTACATG71150.017.5485611
TACATGG70450.017.0005862
TCGTATG6050.016.53478840
ACATGGG72500.016.3294473
Produced by FastQC (version 0.11.5)