FastQCFastQC Report
Mon 6 Feb 2017
SRR2079204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4438759
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51100.036.635241
GGTATCA24750.030.059791
TCAACGC65550.027.9489144
ATCAACG66650.027.7464733
CAACGCA67250.026.9345765
TATCAAC68000.026.921542
AACGCAG70550.025.772516
ACGCAGA82900.021.4324287
GTACATG95450.020.5193311
CGCAGAG86100.020.2754698
TACATGG98050.019.1286872
AGAGTAC81450.018.64453711
ACATGGG104000.017.9476283
CAGAGTA89700.017.12212210
GCAGAGT96600.016.5070539
CATGGGG77750.016.0639114
GTATACG2402.2354288E-715.8600351
GAGTACT43650.015.81217912
GTATAGC11600.015.5120091
GTGTAGC18800.015.4612321
Produced by FastQC (version 0.11.5)