FastQCFastQC Report
Mon 6 Feb 2017
SRR2079204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4438759
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80100.041.53771
GGTATCA31850.037.0292431
TATCAAC110500.029.8283792
ATCAACG117400.028.8396133
TCAACGC117350.028.704854
CAACGCA120800.027.8564785
AACGCAG125450.026.7471286
ACGCAGA146550.022.535177
CGCAGAG149400.022.032268
GAGTACT104350.021.09524712
AGAGTAC144700.020.88776411
AGTACTT112900.019.46296113
CAGAGTA157850.019.2569610
GCAGAGT162850.019.0470779
GTACTTT114800.019.0257914
GTACATG95950.018.8159181
TACATGG98900.017.6580522
CGTGCCG3501.8189894E-1216.75858310
TACTTTT130850.016.63954415
ACTTTTT143150.016.27021616
Produced by FastQC (version 0.11.5)