FastQCFastQC Report
Mon 6 Feb 2017
SRR2079203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4077196
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49150.033.7968441
GGTATCA26000.029.5486771
ATCAACG60950.026.9996513
TCAACGC60700.026.7698354
CAACGCA61750.026.147355
TATCAAC65800.024.8208472
AACGCAG65550.024.6841936
ACGCAGA77200.020.7354837
GTACATG100550.020.0652661
CGCAGAG80450.019.2976888
TACATGG100100.019.1788252
AGAGTAC77050.018.0500811
GTACCGT4000.017.2499896
ACATGGG106300.017.0063653
CAGAGTA83900.016.98770910
GTGTAGC17350.016.9542241
TCGATCG2652.2901077E-916.92098641
GCAGAGT89500.016.271829
GAGTACT34250.015.61696112
GTATATG13750.014.849361
Produced by FastQC (version 0.11.5)